Cytokine balance as a diagnostic marker of inflammation in experimental purulent lesions of soft tissues

Inflammatory process is at the heart of artificial (artificial) purulent-inflammatory diseases soft tissues (APDST), which may lead to development phlegmon and abscesses site invasion within a three-day period. The study cytokine status rats with APDST allows us evaluate dynamics inflammation markers assess peculiarities pathological course in model inflammation. experiment was carried out on 126 white Wistar divided into 3 groups: (1) main group (n = 57), where laboratory used; (2) comparison 58), were injected mixture opportunistic bacterial strains isolated from pure cultures human oral fluid, i.e., S. epidermidis, mitis, salivarius titer 9lg (CFU) per 1 ml together 2.5% hydrocortisone acetate emulsion (20 mg 100 g animal body weight), dexamethasone solution dose 0.5 mg; (3) control 11), animals 0.9% sodium chloride volume 0.3 ml, rate 20 weight, mg. Blood cells studied using Mindray DC-2800 Vet Auto Hematology Analyzer automated rat blood test system. pro-inflammatory cytokines: tumor necrosis factor (TNF), interleukin-1β (IL-1β), interleukin-6 (IL-6), antiinflammatory interleukin-4 (IL-4), interleukin-10 (IL-10) assessed by enzyme immunoassay. To predict towards complications, or resolution inflammatory process, ratio anti-inflammatory cytokines used, normal considered an estimated average for group. When analyzing results this experimental study, nature cellular response dynamics, its relationship determined. All developed tissue 7 days beginning experiment, their mortality 100%. In group, 82.8% cases day 12 15 start without any deaths observed. pro- increased 8-fold already end 1st day, characterized absence significant differences highest levels recorded 15.